Assessment of the zona pellucida microdissection on its thickness in mammalian embryos

The zona pellucida (ZP) is a dynamically changing object that plays an important role during the preimplantation stage of embryogenesis. ZP thickness may affect implantation success and pregnancy rate, it considered as prognostic factor in number studies. study was aimed to assess dynamic changes mouse embryonic after laser assisted hatching (LAH) involved breaching integrity at blastocyst stage. Femtosecond pulses were used perform microsurgery. measured both microsurgery (~Е3.5, i.e. 3.5 days embryogenesis) (~Е5). Significant differences revealed control group embryos: from 6.21 µm (Е3.5) 5.4 (Е5). 6.6 6.2 (Е5) observed subjected LAH non-significant. Tracing particular embryo made possible estimate thinning coefficients experimental groups. findings indicate lower tensile strength case can provide basis for further research on properties using cryopreservation protocols.